DE FINANSIELLA MARKNADERNA--> A B C D E F G 1 2 3 4
Wilo-Yonos PARA - ESBE
Initial screening: In a 1.5 mL Eppendorf tube, KRED (1 mg), rac‐2 (20 m m), DMSO (50 µL or as indicated) and iPrOH (200 µL or as indicated) were added to 900 µL of 100 m m KH 2 PO 4 buffer (1.25 m m MgSO 4, 1 m m NADP +) pH 7.0 or 900 µL of 250 m m KH 2 PO 4 buffer (2 m m MgSO 4, 1.1 m m NADP +, 80 m m d ‐glucose, 10 U/mL glucose dehydrogenase) pH 7.0 in the case of KRED‐101, KRED pH and substrate (2) concentrations for KRED 130 (Codexis) and ketoreducatase from W02011/000693. The better pH for KRED-130 results between 6.5 and 8, being 7 the best value and Km was 30,7 mM, while for ketoreducase from U52011207172 better pH is between 5 and 6, with best results at pH 5,5 and Km 3,4 mM. In a 2.0 mL Eppendorf tube, KRED (2 mg), 3 (27 mM), DMSO (55 μL) and i‐PrOH (165 μL) were added to 900 μL of 125 mM KH 2 PO 4 buffer (1.25 mM MgSO 4, 1 mM NADP +) pH 7.0 or 900 μL of 250 mM KH 2 PO 4 buffer (2 mM MgSO 4, 1.1 mM NADP +, 80 mM d ‐glucose, 10 U/mL glucose dehydrogenase) pH 7.0 in the case of KRED‐101, KRED‐119, KRED‐130, KRED‐NADH‐101 and KRED‐NADH‐110. In 2016, Clayden and co-workers developed a biocatalytic reduction for the atropo-enantioselective synthesis of axially chiral heterobiaryl N-oxides via dynamic kinetic resolution . 44 Particularly, a ketoreductase (KRED 130) was used for the reduction of a configurationally unstable heterobiaryl ketone under mild conditions, while glucose is oxidized to gluconic acid. After screening a commercial kit of reductases, the KRED-130 gave access to the enantiopure (R)-(-ketal-β-hydroxy ester in 86% isolated yield in a 32 mmol scale (Scheme 49). Scheme 49.
146. 147. 148. 151. 151. 152 a 130 kg di ms ha reduce kred åtgärdsm analyser.
Kred. Kreditriskexponering efter avdrag för 130 459. 143 652.
KONVERGENSRAPPORT, MAJ 2012 - European Central Bank
-3 913. 168 247.
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-134 391. -2 572 -1 349. 0 -3 165 oförbrukade externfinansierade medel (kred). Upplupna 130.
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